To maximize the reproducibility of stem-cell-based embryo models across laboratories, SUMO is committed to sharing detailed protocols.

Co-aggregation protocol

Modular aggregoid strategy that spatially couples embryonic and extraembryonic endoderm-like cells to reconstruct key interactions in vitro.

https://doi.org/10.1101/2025.11.09.687163

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Protocol for the Design and Fabrication of the rOoC Platform

The rOoC platform comprises a pump-free, directional-flow recirculating organ-on-a-chip (rOoC) system that generates controlled, unidirectional, gravity-driven flow through the integration of a 3D tilting mechanism and an optimized microfluidic layout.

The platform enables the cultivation and interconnection of two or more tissue or organ representations on-chip, while allowing the incorporation of concentration gradients, endothelial barriers, microvasculature, and circulating cells. These features are integrated within the chip, eliminating the need for external tubing and auxiliary support systems.

https://doi.org/10.1039/d2lc00919f

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To generate more in-depth understanding of Raman chemical signalling, we have developed an autoencoder which predicts biochemical maps within tissues.

A detailed step-by-step protocol how to run RamanSpy and the Autoencoder can be found here:
https://www.science.org/doi/10.1126/sciadv.aec5080
https://github.com/barahona-research-group/dl-raman

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Protocol for generation of trunk-like-structures (& gastruloids)

You can find a detailed step-by-step protocol used by the Veenvliet Lab for the generation of trunk-like-structures and gastruloids (Veenvliet et al., Science 2020) here: https://en.bio-protocol.org/en/bpdetail?id=4042&type=0